Articles

Research Article
Open access

Limb‐bud and heart development (LBH) contributes to glioma progression in vitro and in vivo

  •  26 November 2021

Abstract

Limb-bud and heart (LBH) contributes to glioma progression in vitro and in vivo. Our findings provide new insights into the mechanism by which LBH promotes the development of glioma, improving our understanding of the correlation between LBH with cancer. LBH may have potential as a target for glioma therapy.

Review
Open access

The interplay between pathogens and Atg8 family proteins: thousand‐faced interactions

  •  17 November 2021

Abstract

A possible workflow for the interaction analysis of pathogen proteins and Atg8 family proteins. In this review, we discuss recent advances in the field, the value of prediction software and the research methodology in the study of pathogen-Atg8 family protein interactions, with selected examples of potential LC3-interacting region motif containing SARS-CoV-2 proteins.

Research Article
Open access

Tunneling nanotube formation promotes survival against 5‐fluorouracil in MCF‐7 breast cancer cells

  •  17 November 2021

Abstract

Tunneling nanotubes (TNTs) are F-actin–based tubular extensions that form following stress. Cytotoxic doses of 5-fluorouracil (5-FU) significantly induced TNT formation in MCF-7 cells, which acted as conduits for mitochondrial exchange. The inhibition of TNTs by cytochalasin B increased 5-FU cytotoxicity suggesting that the targeting of TNTs may increase 5-FU efficacy in cancer cells.

Research Article
Open access

Overexpressed Smurf1 is degraded in glioblastoma cells through autophagy in a p62‐dependent manner

  •  15 November 2021

Abstract

In this article, we report that oncogenic SMAD-specific E3 ubiquitin protein ligase 1 is degraded by autophagy in a p62 and E3 ubiquitin ligase activity-dependent manner in glioblastoma cells, a finding that was not previously described. Our research identifies a novel degradation pathway for tumor-related proteins, which has certain guiding significance for tumor treatment.

Research Article
Open access

Characterization of Alzheimer's disease‐like neuropathology in Duchenne's muscular dystrophy using the DBA/2J mdx mouse model

  •  11 November 2021

Abstract

Age-matched D2 mdx mice spend less time exploring the novel object compared with wild-type (WT), suggestive of impaired recognition memory. Biochemical analyses of the D2 mdx brain revealed higher soluble amyloid precursor protein β (APPβ) and APP in the prefrontal cortex of mdx mice compared with WT, and lower soluble APPα in the hippocampus, suggestive of a shift towards amyloidogenesis.

Research Article
Open access

Structural and functional insights into lysine acetylation of cytochrome c using mimetic point mutants

  •  9 November 2021

Abstract

Acetylation is the main post-translational modification of proteins in eukaryotic cells, affecting more than 60% of mitochondrial proteins. Mammalian cytochrome c, in particular, is found acetylated at lysines 8 and 53. Here, we characterize two acetyl-mimetic mutants (Lys-to-Gln mutation) of human cytochrome c to gain insight into the structural and functional changes that could further explain the acetylation role in cancer and other diseases.

Research Article
Open access

Chemical inducer of regucalcin attenuates lipopolysaccharide‐induced inflammatory responses in pancreatic MIN6 β‐cells and RAW264.7 macrophages

  •  9 November 2021

Abstract

Derrisfolin A, a chemical inducer of regucalcin, attenuated lipopolysaccharide-induced inflammatory responses in pancreatic MIN6 β-cells. Regucalcin in MIN6 cells and RAW264.7 macrophages also blocks inflammatory interaction between both cells in a transwell coculture system. Regucalcin might act as a suppressor of macrophage-associated pancreatic β-cell inflammation.

Research Article
Open access

Cpeb1 expression is post‐transcriptionally regulated by AUF1, CPEB1, and microRNAs

  •  8 November 2021

Abstract

We have previously shown that loss of interaction between AREs in the Cpeb1 3’UTR and AUF1, an mRNA decay factor, increased Cpeb1 mRNA levels but reduced the protein levels (Oe S. et al. 2021, Biochem Biophys Res Commun 534, 491-497). In this study, we revealed that Cpeb1 expression is post-transcriptionally regulated by multiple inhibitory machineries that are regulated by AUF1, CPEB1, and microRNAs.

Research Article
Open access

Identification and characterization of a novel glutaminase inhibitor

  •  8 November 2021

Abstract

Discovery and characterization of the novel glutamine (GLS) inhibitor C19 by high-throughput screening from Enamine. C19 inhibits activity of GLS1 and reduces proliferation and cytokine secretion from activated CD4+ T cells.

Review
Open access

Bacterial phospholipases C with dual activity: phosphatidylcholinesterase and sphingomyelinase

  •  8 November 2021

Abstract

The aim of this review is to discuss similarities and differences among the most explored bacterial enzymes that have both phospholipase C and sphingomyelinase C activities. The structural aspects of three of those enzymes, their substrate specificity, and in silico substrate docking analysis are discussed, as well as their mechanisms of toxicity in cultured cells and animal models.

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Method
Open access

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer

Abstract

Direct amplification of 16S ribosomal RNA genes combined with MinION™ sequencing provides an attractive option for rapid detection of bacteria. Mechanical cell disruption preceding direct PCR is indispensable for obtaining an accurate representation of the bacterial composition. Our simple workflow for rapid bacterial identification reduces the turnaround time from sample to result and provides a reliable method applicable to clinical settings.

Research Article
Open access

iPSC screening for drug repurposing identifies anti‐RNA virus agents modulating host cell susceptibility

Abstract

FDA-approved drugs, identified by iPSC screening with Sendai virus, exhibited inhibitory effects on the infection of Huh7 cells by Ebola virus and of Vero E6 cells by SARS-CoV-2. These drugs, with efficacy in different combinations of RNA viruses and cells, may have therapeutic potential for RNA virus infections, including newly emerging and mutated viruses, by modulating host cell susceptibility.

Posters
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Posters

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Research Article
Open access

The CAG promoter maintains high‐level transgene expression in HEK293 cells

Abstract

Through flow cytometry and qPCR technology, the promoter vector for high expression of eGFP protein in HEK293F cells was optimized and verified with hepatitis B surface antigen. The CAG promoter vector can not only effectively increase and maintain the expression level of recombinant proteins, but also increase the expression of transgenes under low temperature conditions.

Research Article
Open access

Seeded assembly in vitro does not replicate the structures of α‐synuclein filaments from multiple system atrophy

Abstract

The assembly of certain proteins into amyloids underlies multiple neurodegenerative diseases. The spreading of these assemblies through the brain is thought to occur through a prion-like mechanism. We used filaments extracted from multiple system atrophy brains to seed recombinant α-synuclein. The resulting structures differ from those of the seeds, indicating that seeded assembly does not necessarily replicate the seed structures.

Research Article
Open access

Potential anti‐SARS‐CoV‐2 drug candidates identified through virtual screening of the ChEMBL database for compounds that target the main coronavirus protease

Abstract

Here, I describe virtual screenings for compounds targeting main protease of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to predict potential anti-SARS-CoV-2 drugs and lead compounds. The procedure described herein may be a suitable drug repositioning strategy for the discovery of anti-SARS-CoV-2 drugs from drug libraries.

Research Article
Open access

RDM1 promotes neuroblastoma growth through the RAS–Raf–MEK–ERK pathway

Abstract

High RAD52 motif containing 1 (RDM1) levels are shown to be associated with poor clinical outcomes in neuroblastoma (NB). Disruption of RDM1 suppresses NB cell proliferation. RDM1 promotes cell proliferation via the RAS–Raf–MEK–ERK signaling pathway.

Research Article
Open access

Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self‐cleavage exhibits enhanced enzymatic activity

Abstract

The use of tobacco etch virus (TEV) protease is limited by its poor solubility and self-inactivation. We analyzed the effect of quadruple mutations (T17S, N68D, I77V, and S219X), which affect solubility and inhibit autoinactivation in TEV protease. In a background of T17S, N68D, and I77V mutations, soluble S219N mutant TEV protease serves as a more efficient catalyst than soluble S219V mutant.

Review Article
Open access

The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability

Abstract

Biotechnology holds the power to boost the transition from fossil-based to bio-based plastics by offering new sustainable routes for polymer synthesis based on waste biomasses.

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