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Corrigendum
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Corrigendum

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Original Article
Open access

TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1

Abstract

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The ubiquitin E3 ligase TRIM27 is a multifunctional protein implicated in several cellular processes including innate immunity, endosomal recycling, cancer development and apoptosis. Here we show that TRIM27 forms ubiquitylated cytoplasmic bodies that associate with autophagy receptor SQSTM1/p62 and the kinase TBK1. Crowding of TBK1 leads to its activation by autophosphorylation. This TRIM27-pTBK1-SQSTM1/p62 pathway induces clustering of mitochondria and enhances their degradation via mitophagy.

Original Article

KRIT1-mediated regulation of neutrophil adhesion and motility

Abstract

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Loss of KRIT1 expression in neutrophils has no effect on adhesion to and migration on ICAM-1 but causes a loss of the neutrophil attachment and spreading of fibronectin and a reduction in fibronectin-binding integrin activity. This may explain why, though KRIT1-deficient neutrophils migrate faster ex vivo, migration of KRIT1−/− neutrophils into the lung in response to lipopolysaccharide was decreased. Thus, we conclude that KRIT1 regulates neutrophil behaviour.

Commentary
Open access

New links for meprin β within the protease web

Abstract

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Proteases do not act alone but in cascades, circuits and networks that together form the protease web. Disturbances of the protease web can result in disease, but its complex connections have been only poorly unravelled. Werny et al. have identified new links for meprin β within the protease web, helping to better understand the high connectivity of this sheddase with other proteases and its roles in tissue inflammation and fibrosis.

Comment on: https://doi.org/10.1111/febs.16586

Original Article

Only one carbon difference determines the pro-apoptotic activity of α-tocopheryl esters

Abstract

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Tocopheryl succinate (TS) and Tocopheryl glutaate (Tglu) are the esters of α-Tocopherol. TS consists of four carbon atoms in the terminal di-carboxylic moiety, while Tglu consists of five carbon atoms. Interestingly, structural difference by only one carbon oppositely affects biological activities, which explains the opposite role of TS and Tglu in apoptosis induction. We propose that tocopheryl esters are versatile in biological activities that can be explored in the future.

Original Article
Open access

The endoglycosidase activity of Dispersin B is mediated through electrostatic interactions witcationic poly-β-(1→6)-N-acetylglucosamine

Abstract

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Dispersin B is a biofilm dispersing hexosaminidase enzyme that breaks down poly-β-(1→6)-N-acetylglucosamine (PNAG) polysaccharides present in the biofilm matrix of Gram-positive and Gram-negative bacteria by catalyzing both exo- and endo-glycosidic bond cleavage of PNAG. We demonstrate that the endoglycosidase activity is mediated through electrostatic interactions between D242 and cationic glucosamine in the –2 binding site, and that endoglycosidase activity is required for efficient dispersal of Staphylococcus epidermidis biofilms.

Original Article
Open access

Reciprocal abrogation of PKM isoforms: contradictory outcomes and differing impact of splicing signal on CRISPR/Cas9 mediates gene editing in keratinocytes

Abstract

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Nullification of PKM1 expression augments glutaminolysis and lactate production. Inactivating the splicing of exon 10 and PKM2 expression results in enhanced level of PKM1 protein, but otherwise no remarkable metabolic changes (right). The inactivation of exon 9 leads to the expression of PKM2 alone, which forces to ‘glutaminolysis addicted’ and increased lactate secretion with vast cellular changes (left).

Original Article

In-solution structure and oligomerization of human histone deacetylase 6 – an integrative approach

Abstract

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Histone deacetylase 6 (HDAC6) is a multidomain, zinc-dependent cytosolic hydrolase that acts on numerous non-histone substrates. Due to its complex domain organization, HDAC6 is a challenging target for X-ray crystallography. Here, we used an integrative structural biology approach to build an in-solution model of full-length human HDAC6 by combining experimental data from several orthogonal biophysical techniques complemented by molecular modelling. Our data further provide insights into concentration-dependent HDAC6 oligomerization.

Original Article
Open access

Unexpected expansion of the voltage-gated proton channel family

Abstract

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Voltage-gated ion channels, whose first identified function was to generate action potentials, are divided into subfamilies with numerous members. To date, all species found to express HV have exclusively one gene that codes for this unique ion channel. Here we report the discovery and characterization of three proton channel genes in the classical model system of neural plasticity, Aplysia californica. Each channel (AcHV1, AcHV2, AcHV3) has distinct biophysical properties.

Original Article
Open access

The intramembrane proteases SPPL2a and SPPL2b regulate the homeostasis of selected SNARE proteins

Abstract

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Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins facilitate membrane fusion events. We screened 18 SNARE proteins with a tail-anchored topology for cleavage by the intramembrane proteases signal peptide peptidase-like (SPPL) 2a and b. VAMP1-4 were processed by SPPL2a/b. Loss of these proteases in mice leads to accumulation of VAMP1-4 in a cell type- and tissue-dependent manner. Thus, SPPL2a/b control cellular levels of VAMP1-4 by initiating the degradation of these SNAREs.

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