A common SNP in the UNG gene decreases ovarian cancer risk in BRCA2 mutation carriers

Single nucleotide polymorphisms (SNPs) in DNA glycosylase genes involved in the base excision repair (BER) pathway can modify breast and ovarian cancer risk in BRCA1 and BRCA2 mutation carriers. We previously found that SNP rs34259 in the uracil‐DNA glycosylase gene (UNG) might decrease ovarian cancer risk in BRCA2 mutation carriers. In the present study, we validated this finding in a larger series of familial breast and ovarian cancer patients to gain insights into how this UNG variant exerts its protective effect. We found that rs34259 is associated with significant UNG downregulation and with lower levels of DNA damage at telomeres. In addition, we found that this SNP is associated with significantly lower oxidative stress susceptibility and lower uracil accumulation at telomeres in BRCA2 mutation carriers. Our findings help to explain the association of this variant with a lower cancer risk in BRCA2 mutation carriers and highlight the importance of genetic changes in BER pathway genes as modifiers of cancer susceptibility for BRCA1 and BRCA2 mutation carriers.

Single nucleotide polymorphisms (SNPs) in DNA glycosylase genes involved in the base excision repair (BER) pathway can modify breast and ovarian cancer risk in BRCA1 and BRCA2 mutation carriers. We previously found that SNP rs34259 in the uracil-DNA glycosylase gene (UNG) might decrease ovarian cancer risk in BRCA2 mutation carriers. In the present study, we validated this finding in a larger series of familial breast and ovarian cancer patients to gain insights into how this UNG variant exerts its protective effect. We found that rs34259 is associated with significant UNG downregulation and with lower levels of DNA damage at telomeres. In addition, we found that this SNP is associated with significantly lower oxidative stress susceptibility and lower uracil accumulation at telomeres in BRCA2 mutation carriers. Our findings help to explain the association of this variant with a lower cancer risk in BRCA2 mutation carriers and highlight the importance of genetic changes in BER pathway genes as modifiers of cancer susceptibility for BRCA1 and BRCA2 mutation carriers.

Introduction
Women carrying germline mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of developing breast, ovarian, and other cancers (Milne et al., 2008). However, mutation carriers show considerable differences in disease manifestation, and this suggests the existence of other genetic or environmental factors that modify the risk of cancer development. BRCA proteins are involved in double-strand break (DSB) DNA repair through the homologous recombination pathway (O'Donovan and Livingston, 2010), and cells harboring mutations in these genes are dependent on other DNA repair mechanisms. In this regard, we have shown that single nucleotide polymorphisms (SNPs) in genes from the base excision repair (BER) pathway can modify breast or ovarian cancer susceptibility in BRCA1 and BRCA2 mutation carriers (Osorio et al., 2014).
The BER pathway corrects base lesions that result from deamination, oxidation, or methylation (Xue et al., 2016). BER is initiated by DNA glycosylases that cleave the N-glycosylic bond between the sugar and the base, and release the damaged base to form an abasic site, also termed an apurinic/apyrimidinic (AP) site (Maynard et al., 2009). A deficiency in BER can give rise to an accumulation of DSBs, which in the presence of a defective BRCA1 or BRCA2 background can persist and lead to cell cycle arrest or cell death. A synthetic lethal interaction was described between the BRCA1/2 genes and poly(ADP-ribose) polymerase (PARP1) involved in the BER pathway, with BRCAdeficient cells being extremely sensitive to PARP1 inhibitors (Bryant et al., 2005;Farmer et al., 2005).
On the other hand, the BER pathway is essential for maintaining telomere integrity in mammals (Jia et al., 2015). Telomeres are susceptible to uracil misincorporation, which is primarily recognized and removed by the uracil-DNA glycosylase (UNG) (Cortizas et al., 2016). Due to the presence of long arrays of TTAGGG repeats, uracil can appear in telomeric DNA by misincorporation of deoxyuridine triphosphate (dUTP) instead of deoxythymidine triphosphate (dTTP) opposite adenine or by deamination of cytosine to uracil opposite guanine (Krokan et al., 2002). Accumulation of uracil interferes with telomere homeostasis, and UNG-initiated BER is necessary for the preservation of telomere integrity (Vallabhaneni et al., 2015).
In view of the above, we hypothesized that SNPs in DNA glycosylase genes might interfere with telomere maintenance and thus contribute to the risk of developing cancer. Supporting this idea, we reported that variant rs2304277, located in the 3 0 -UTR of the glycosylase gene OGG1, is associated with higher ovarian cancer risk in BRCA1 mutation carriers, probably due to transcriptional downregulation of OGG1 and increased DNA damage and telomere instability (Ben ıtez-Buelga et al., 2016). Similarly, we analyzed variant rs804271, previously associated with increased breast cancer risk in BRCA2 mutation carriers (Osorio et al., 2014), which is located within the promoter region of the glycosylase gene NEIL2. The modifier effect of this variant may be due to its negative impact on the performance of the NEIL2 enzyme, leading to an accumulation of oxidative lesions at telomeres (Ben ıtez-Buelga et al., 2017).
In the present study, we aimed to explain the molecular basis of the protective effect exerted by a SNP located in the 3 0 -UTR of the UNG gene (rs34259) in BRCA2 mutation carriers (Osorio et al., 2014). For that purpose, we explored the effects of the SNP on UNG activity and expression levels, and its possible involvement in telomere integrity.

Patients and healthy controls
The study was performed in accordance with the principles of the Declaration of Helsinki. All patients and controls signed an appropriate informed consent form, and the proposal was approved by the ethics committee at the Fuenlabrada University Hospital, Madrid, Spain.
We studied a familial breast and ovarian cancer (FBOC) series of 344 individuals from 173 families meeting high-risk criteria, and screened for deleterious mutations in the BRCA1 and BRCA2 genes, as reported previously (Milne et al., 2008). Thirty-two families carried a deleterious mutation in BRCA1, 31 in BRCA2, and 110 did not carry any mutation in either of these two genes (BRCAX families). One hundred eleven controls were included who were relatives of BRCA1/2 mutation carriers, did not have personal cancer antecedents, and did not harbor the corresponding familial mutation in the BRCA1 or BRCA2 genes. The different traits studied in this series are detailed in Table 1.

DNA extraction and genotyping of SNP rs34259
DNA was extracted from peripheral blood of FBOC patients using the Maxwell Ò FSC Instrument (Promega, Madison, WI, USA) following the manufacturer's instructions and quantified by the PicoGreen Ò fluorometric assay (Thermo Fisher Scientific, Waltham, MA, USA). Single nucleotide polymorphism genotyping was carried out using a KASPar probe specifically designed for rs34259 (LGC Genomics, Berlin, Germany). Allelic discrimination assays were performed in duplicate using the 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) and the Abi QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) following the instrument-specific conditions detailed by the manufacturer (LGC Genomics).

RNA expression analysis
RNA was extracted from peripheral blood mononuclear cells using TRIzol Ò Reagent (Thermo Fisher Scientific). RNA quantity and quality were assessed by NanoDrop Ò (ND-1000 V3.7.1; Thermo Fisher Scientific). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) was utilized for cDNA synthesis following the manufacturer's instructions.
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase (Nilsen et al., 1997). We designed specific primers to quantify total UNG mRNA expression and the relative expression of each isoform. Two microliters of cDNA at a final concentration of 10 ngÁlL À1 was mixed with GoTaq Ò qPCR MasterMix 19 (Promega) and 1 lM cDNA primers of each pair of primers (F/R) in a final volume reaction of 10 lL. Primers used are listed in Table S1. The amplification conditions consisted of an initial step at 95°C for 10 min, followed by 40 cycles of 10 s at 95°C and 1 min at 65°C. Each qPCR was performed in triplicate including no-template controls in an Abi QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative UNG/UNG1/UNG2 mRNA expression was calculated using the 2 DDCt method for qPCR analysis after normalization with the housekeeping gene GAPDH using the QUANTSTUDIO TM Real-Time PCR Software (Applied Biosystems).

Western blotting
The expression of UNG1 was quantified by western blot analysis in a subset of controls (n = 10) and BRCA2 mutation carriers (n = 20) from the FBOC series. Briefly, peripheral blood mononuclear cells were isolated from whole blood using TRIzol Ò Reagent (Thermo Fisher Scientific) according to manufacturer's instructions. Cell lysates were prepared in RIPA buffer (Sigma-Aldrich, San Luis, MO, USA) in the presence of a protease inhibitor cocktail (Roche, Basel, Switzerland). Total protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) following the manufacturer's instructions. Sixty micrograms of protein was analyzed by SDS/PAGE and transferred to Immobilon-FL membranes (Millipore, Burlington, MA, USA). Membranes were blocked in TBS-T (50 mM Tris/HCl, 150 mM NaCl, pH 7.5 plus 0.2% Tween-20) and 5% nonfat milk for 1 h at RT. Blots were probed with the following primary antibodies: mouse anti-UNG (#TA503563; OriGene, Rockville, MD, USA) at 1/1000 dilution and mouse anti-actin (A2228; Sigma-Aldrich) at 1/10 000 dilution in TBS-T containing 5% nonfat milk. Antimouse IgG-HRP (Dako, Glostrup, Denmark) was used as the secondary antibody, and the immunoblots were developed using Immobilon Classico Western HRP substrate (Millipore). Each western blot was performed in quadruplicate. Images were analyzed using IMAGEJ software (NIH Image, Bethesda, MD, USA), and UNG1 protein level was normalized by actin.
In parallel, given that UNG2 protein levels in peripheral blood mononuclear cells from the FBOC series were too low to analyze their relative expression, we also performed western blot analyses of a previously described set of 18 lymphoblastoid cell lines (LCLs) (Vaclov a et al., 2015) proceeding from BRCA1 mutation carriers and controls following the same protocol.

Oxidative DNA damage within telomeres
We used a qPCR-based method previously described to evaluate the accumulation of oxidative lesions within telomeric DNA based on differences in PCR kinetics between template DNA digested by formamidopyrimidine-DNA glycosylase (FPG) and undigested DNA (O'Callaghan et al., 2011). Incubation and qPCR amplification of genomic DNA were performed as described by O'Callaghan et al. (2011) to estimate oxidative DNA damage levels at telomeres and the 36B4 locus.

Quantification of uracil accumulation at telomeres
The telomere oxidation protocol (O'Callaghan et al., 2011) can be adapted to quantify the accumulation of different base lesions by incubating the DNA with other glycosylases that are sensitive to other specific base lesions. We used UNG to measure the accumulation of uracil, which is recognized and excised by this enzyme (Hegde et al., 2008), at telomeres.
Due to the high affinity of UNG for DNA (Zharkov et al., 2010), we optimized the protocol using a low UNG concentration and decreasing DNA amounts and incubation times. One hundred and eighty nanograms of genomic DNA was incubated in the absence or presence of 130 nM UNG (provided by T. Helleday, Karolinska Institutet, Stockholm, Sweden) in reaction buffer (25 mM Tris/HCl pH 8.0, 15 mM NaCl, 2 mM MgCl 2 , and 0.0025% Tween-20) for 30 min at 37°C. The reaction was stopped by incubation at 95°C for 5 min. qPCR analysis was performed on 10 ng of digested or undigested genomic DNA using the same reagents and conditions as described in the original protocol for FPG (O'Callaghan et al., 2011).

Immunodetection of oxidized proteins
Oxidized proteins in plasma samples were detected by measuring the levels of carbonylated proteins as previously described (Garc ıa-Gim enez et al., 2012). Carbonylated proteins are a widely used biomarker of chronic oxidative stress (Fedorova et al., 2013).

Telomere length measurement
Telomere length (TL) was quantified by high-throughput quantitative fluorescence in situ hybridization (HT-QFISH) with automated fluorescence microscopy as previously described (Canela et al., 2007). Because TL is strongly heritable (Pooley et al., 2013), BRCA status, the presence or absence of the SNP, and TL were assessed in the same member of each family. Whenever possible, we used the index case, and if this sample was not available, we used the most recently genotyped individual. As we previously demonstrated that chemotherapy affects TL (Ben ıtez-Buelga et al., 2015), we excluded patients from the analysis who were undergoing this treatment.

Telomerase assay
Protein extracts were obtained from peripheral blood mononuclear cells cultured in RPMI supplemented with 20% fetal bovine serum and phytohemagglutinin during 4-5 days, according to the recommendations of the manufacturer of the TRAPeze telomerase detection kit (Millipore). The average telomerase activity was determined in each sample using 0.5, 0.25, and 0.125 lg of protein extract and normalized with the internal control included in the assay. Because telomerase activity can be affected by chemotherapeutic agents (Ben ıtez-Buelga et al., 2015), we excluded all patients who received chemotherapy at any time during their lifetime.

Statistical analysis
To evaluate the effect of the SNP for each of the studied variables, we considered heterozygotes and homozygotes (GC/CC) as a single group, as the cancer modifier effect of rs34259 acts in a dominant fashion in BRCA2 mutation carriers (Osorio et al., 2014).
Pearson's chi-squared test was used to calculate whether the frequency of the SNP among the FBOC groups was significantly different from the frequency reported in the 1000 Genomes Project for the Iberian subpopulation (Zerbino et al., 2018). The Spearman correlation test was used to establish whether correlations between variables were statistically significant. We performed linear regression analysis to test whether cancer antecedents in BRCA1 and BRCA2 mutation carriers were associated with any of the variables evaluated in this study, but we did not find significant differences (P < 0.05) between healthy BRCA1 and BRCA2 carriers or cancer cases. Hence, we did not stratify for cancer status in these groups (Table S2).
The Kolmogorov-Smirnov test was used to evaluate whether the data sets were normally distributed. For comparative analyses, statistically significant differences were assessed by an unpaired t-test for normal distributions and the Mann-Whitney U-test for nonnormal distributions. Linear regression analysis including the UNG SNP as explanatory variable was run to test whether this SNP affected the variables studied. The effect size of the studied variant was defined as the slope of the linear regression line and was computed as the effect of the alternative allele (C) relative to the reference allele (G).
Statistical calculations and graphs were done using the SPSS software package version 19.0 (IBM, Armonk, NY, USA) and GRAPHPAD PRISM 5.03 (GraphPad Software Inc, San Diego, CA, USA). In all analyses, a 2tailed P value < 0.05 was considered statistically significant.

Association study, validation, and fine mapping
In a previous study, using a tagging SNP approach in a large series of BRCA1 and BRCA2 mutation carriers (n = 23 463) from the CIMBA consortium, we found that SNP rs34259 showed the strongest association with ovarian cancer risk among all SNPs covering the UNG gene (tagged or imputed): HR: 0.80, 95% CI: 0.69-0.94, P = 7.6 9 10 À3 (Osorio et al., 2014). This association was confirmed in a larger series of BRCA2 mutation carriers (4291 new cases) from the OncoArray Consortium (Amos et al., 2017) (HR: 0.84, P = 7.6 9 10 À3 ). SNP rs34259 is located in the 3 0 UTR of the UNG gene, 2.4 kb downstream of the translation termination codon. Using HAPLOREG v4.1 (Ward and Kellis, 2012), we were not able to detect a more plausible causal SNP among those in high linkage disequilibrium with rs34259 according to their predicted regulatory features (Table S3). Indeed, rs34259 has been identified as a trans expression quantitative trait locus (eQTL) SNP that decreased UNG gene expression in two independent eQTL studies Westra et al., 2013) and we considered it the best candidate.
We genotyped SNP rs34259 in the FBOC sample set to evaluate its association with the studied variables. Genotype distributions were in Hardy-Weinberg equilibrium (v 2 = 0.03; P = 0.86). The different groups of cases and controls presented similar genotype and allele frequencies, not statistically different from the frequencies reported in the 1000 Genomes Project for the Iberian subpopulation (Zerbino et al., 2018) (Table S4).

rs34259 is associated with lower UNG mRNA and protein levels
We first analyzed the SNP effect on transcriptional regulation in different tissues using the GTEx eQTL web server (Carithers et al., 2015). We found significantly decreased UNG mRNA levels associated with SNP rs34259 in several tissues, including breast (effect size = À0.17; P = 0.023) and blood (effect size = À0.19; P < 0.0001; Table S5).
In parallel, we analyzed UNG mRNA levels in the FBOC series considering the BRCA status and the presence or absence of the UNG variant (Fig. 1A). First, we confirmed in a subgroup of samples (n = 97) that the mRNA levels of both UNG isoforms (UNG1 and UNG2) were highly correlated (r = 0.551; P < 0.001) and that total UNG mRNA expression was correlated with each of the two isoforms and, therefore, representative of both (Fig. S1). We detected significantly lower UNG mRNA expression in individuals harboring the variant (effect size = À0.209; P < 0.001). The effect was more pronounced in the BRCA2 group (effect size = À0.366; P = 0.007) and remained significant when analyzing both isoforms separately (Fig. S2).
Given that the SNP protective effect is for ovarian cancer, we also determined UNG mRNA expression in tissues of 17 prophylactic oophorectomies from BRCA1 and BRCA2 mutation carriers. In this cohort, we also found a trend toward lower total UNG mRNA expression associated with the studied SNP (P = 0.056; Fig. S3), which was significant for the UNG1 isoform (P = 0.045).
To confirm whether this downregulation was translated into lower expression of the protein, we determined UNG1 protein expression by western blotting (WB) in 10 controls, of which 4 were carriers of the SNP, and in 20 BRCA2 carriers, of which 10 were carriers of the SNP (Fig. 1B). Quantification showed that BRCA2 carriers harboring SNP rs34259 had lower UNG1 protein levels (P = 0.023) when controls and BRCA2 carriers were combined, and the effect of SNP rs34259 on UNG1 protein levels remained significant (P = 0.021; Fig. 1C). UNG1 mRNA levels correlated significantly with UNG protein levels in these patients (r = 0.791; P < 0.001) (Fig. 1D). Finally, we performed WB of both UNG1 and UNG2 in a set of 18 LCLs and confirmed that both UNG isoforms remained highly correlated at the protein level (r = 0.829; P < 0.001; Fig. S4A,B). Despite the reduced sample size, we also found a trend toward lower UNG1 and UNG2 protein levels in the LCL series associated with the UNG variant (Fig. S4C).

Accumulation of DNA damage at the telomeres
We analyzed the accumulation of two kinds of lesions: 8-oxoguanine and uracil, which are detected by FPG and UNG glycosylases, respectively.

SNP rs34259 is associated with lower oxidative DNA damage
When analyzing the accumulation of 8-oxoguanine, we observed significantly lower oxidation levels in individuals harboring the variant (P = 0.008) ( Fig. 2A). We were not able to detect significant differences within each mutational group. However, a statistically significant lower oxidative DNA damage associated with the SNP was found in controls (P = 0.009), suggesting that the SNP is associated with lower oxidative damage accumulation at telomeres independently of the BRCA status.

BRCA2 mutation carriers harboring SNP rs34259 show lower uracil accumulation at the telomeres
After treatment with UNG, telomeric DNA showed an average decrease of 54% in PCR amplification compared to a 22% decrease observed when amplifying the 36B4 control locus (P < 0.0001), reflecting a predominant presence of uracil in telomeres (Fig. S5).
We did not find significant differences in uracil levels at telomeres among BRCA groups or controls. However, when we stratified according to the SNP (Fig. 2B), we detected a significantly lower uracil accumulation at telomeres when the variant was present for BRCA2 mutation carriers (P = 0.01). This result suggests that the protective effect for ovarian cancer risk associated with SNP rs34259 in BRCA2 mutation carriers could be due to an increased UNG activity, leading to less accumulation of uracil at the telomere region.

Lower protein carbonylation level in individuals harboring SNP rs34259
No significant differences were found in carbonylation levels in relation to the BRCA status. Notwithstanding, we found a trend toward lower carbonylation levels in all FBOC individuals with the variant (P = 0.052). In addition, for BRCA2 mutation carriers harboring the SNP we detected a significantly lower carbonylation level (P = 0.016) (Fig. 3). These results suggest that the SNP in UNG is associated with lower oxidative stress susceptibility that becomes pronounced in BRCA2 mutation carriers.

Shorter telomeres in BRCA2 mutation carriers harboring the SNP
We first evaluated TL distribution in 91 healthy women as a function of age to obtain a regression line to adjust TL in the FBOC samples. As expected, we found a decrease in TL with age (Fig. S6). When the effect of rs34259 was analyzed for each BRCA mutation group, we only found a significant effect among BRCA2 mutation carriers: In this group, SNP carriers had a reduced age-adjusted TL (P = 0.018; Fig. 4A) and showed a trend toward accumulation of short telomeres (P = 0.067; Fig. 4B).

Telomerase activity
We found a significant correlation between telomerase activity and telomere length (r = 0.313; P < 0.001). Mean telomerase activity was lower when the SNP was present in all groups, but it did not reach statistical significance (Fig. S7).

Discussion
The SNP rs34259 in the 3 0 UTR of the UNG gene may decrease ovarian cancer risk in BRCA2 mutation carriers (Osorio et al., 2014). However, the molecular mechanism underlying this association is unknown. In the present report, we show that rs34259 is associated with significant UNG downregulation and with lower levels of oxidative DNA damage at telomeres. In addition, we found that for BRCA2 mutation carriers the SNP is associated with significantly lower oxidative stress susceptibility and lower uracil accumulation at telomeres.
As it has been previously demonstrated that the region where the variant is located is a potential seed  site for microRNAs that downregulate UNG expression (Hegre et al., 2013), we decided to explore the effect of this SNP on UNG mRNA and protein levels.
We detected significantly lower UNG mRNA and UNG1 protein levels associated with SNP rs34259, which became pronounced in BRCA2 mutation carriers. It has been shown that overexpression of human UNG in yeast causes DNA damage due to the generation of AP sites faster than they are repaired (Elder et al., 2003). In this regard, the lower UNG expression associated with rs34259 may prevent AP repair from becoming saturated, and this may in part explain its protective effect. Given the dominant role of UNG for processing uracil at telomeres (Cortizas et al., 2016), we evaluated uracil accumulation and observed that this was higher in telomeric DNA than in other genomic regions (Fig. S5), confirming that telomeres are prone to uracil accumulation (Vallabhaneni et al., 2015). When we analyzed the SNP effect, we found significantly lower uracil accumulation when the SNP was present, but only for BRCA2 carriers (Fig. 2B). This suggests that rs34259 could have a positive impact on UNG enzyme performance that may help to explain the protective effect of this SNP in BRCA2 carriers.
Furthermore, we explored the impact of this SNP on other features related to telomere biology, such as oxidative damage. Because telomeres are especially susceptible to DNA oxidation (O'Callaghan et al., 2011;Von Zglinicki et al., 2000), we evaluated the accumulation of 8-oxoguanine as a measure of oxidative damage. We observed significantly lower 8-oxoguanine levels in individuals harboring the variant ( Fig. 2A), suggesting that the SNP is associated with lower oxidative DNA damage accumulation at the telomeres.
We found that the SNP impact on UNG expression affects both nuclear (UNG2) and mitochondrial (UNG1) isoforms (Fig. S2). Therefore, apart from the telomeres, it is probable that mitochondrial DNA of patients harboring the SNP presents lower damage, given that oxidative base lesions in mitochondria are repaired by UNG1 (Akbari et al., 2007). In addition, we analyzed whether the lower levels of oxidative DNA damage associated with the SNP could be related to lower chronic oxidative stress susceptibility. We found lower protein carbonylation levels when rs34259 was present (Fig. 3), and this was more pronounced in BRCA2 mutation carriers. These results suggest that the SNP in UNG is associated with lower oxidative stress susceptibility, especially for BRCA2 carriers. Oxidative stress plays an important role in the development and progression of cancer (Valko et al., 2006), and therefore, the lower oxidative stress associated with the SNP may help to explain the lower cancer risk of BRCA2 carriers that harbor the SNP. We also found a significantly shorter TL associated with the SNP in carriers of BRCA2 mutations (Fig. 4A). TL is regulated by the shelterin protein complex that protects telomeres (De Lange, 2005) and by telomerase, a ribonucleoprotein complex that adds TTAGGG repeats to the chromosome ends (Blackburn, 2001). Our data reflect this expected positive correlation between TL and telomerase activity. The accumulation of uracil in telomeres weakens the binding affinity of the shelterin component POT1, increasing the accessibility of telomerase. Thus, UNG deficiency causes defective uracil removal that can lead to lengthening of telomeres, as has been demonstrated in mice (Vallabhaneni et al., 2015). According to this model, the short telomeres phenotype observed in BRCA2 carriers harboring the SNP could be due to the lower uracil accumulation at telomeres, also associated with this group, which facilitates shelterin binding.

Conclusions
We have found that the ovarian cancer risk modifier SNP rs34259 may have a positive impact on UNG enzyme performance and is associated with lower oxidative levels in BRCA2 carriers, which may explain the cancer-protective effect attributed to this SNP in this group. Taken together, our findings support the importance of genetic changes in BER pathway genes as modifiers of cancer susceptibility for BRCA1 and BRCA2 mutation carriers.

Supporting information
Additional supporting information may be found online in the Supporting Information section at the end of the article. Fig. S1. (A) Correlation analysis between total UNG mRNA expression and UNG1 mRNA expression. (B) Correlation analysis between total UNG mRNA expression and UNG2 mRNA expression. (C) Correlation analysis between UNG1 mRNA and UNG2 mRNA expression. Spearman's test was used to assess the significance of the correlations. Fig. S2. Expression levels of specific isoforms of UNG mRNA according to the presence or absence of the SNP (noncarriers (GG)/carriers (GC/CC)). Fig. S3. Expression levels of specific isoforms of UNG mRNA according to the presence or absence of the SNP (noncarriers (GG)/carriers (GC/CC)) in ovarian tissue from BRCA1 and BRCA2 patients (n = 17).  (Vaclov a et al., 2015). Briefly, the LCLs were established by Epstein-Barr virus transformation of peripheral blood lymphocytes from eleven healthy women carrying heterozygous mutations in BRCA1 and seven noncarrier relatives (controls). None of the women included in the study had personal antecedents of cancer. Cells were cultured in RPMI-1640 media (Sigma-Aldrich) supplemented with 20% non-heatinactivated fetal bovine serum (Sigma-Aldrich), penicillin-streptomycin (Gibco) and Fungizone (Gibco). Cells were cultured at 37°C in a 5% CO 2 atmosphere and were maintained in exponential growth by daily dilution to 10 6 cellsÁmL À1 complete media. Protein extraction and western blotting were performed as described in the Materials and Methods section. (B) Correlation analysis between UNG1 and UNG2 protein expression levels in LCLs. Spearman's test was used to assess the significance of the correlation. (C) UNG1 and UNG2 expression levels in the LCL series according to the presence or absence of the SNP (noncarriers (GG)/carriers (GC/CC)). Bars show the mean and the standard error of the mean (SEM). Numbers in brackets indicate sample size. Unpaired t-tests were performed for statistical significance. DNA extraction and SNP genotyping were performed as are described in the Materials and Methods section. Fig. S5. PCR amplification efficiency at the untreated and UNG-treated telomeric and 36B4 loci. Fig. S6. Telomere length (TL) distribution in peripheral blood leukocytes as a function of age for the control population (n = 91), measured by HT QFISH. Fig. S7. Comparative analysis of telomerase activity in the FBOC series according to the presence or absence of the UNG SNP (noncarriers (GG)/carriers (GC/ CC)). Table S1. Primers used for UNG RNA expression analysis. Table S2. Linear regression analysis in BRCA 1/2 mutation carriers. Table S3. Variants within the block of linkage disequilibrium (LD) > 0.8 with SNP rs34259. Table S4. Frequency distribution of the UNG variant rs34259 among FBOC groups. Table S5. Summary of information in the GTEx eQTL server regarding transcriptional downregulation of UNG in 16 different tissues when rs34259 is present.