A New Polynucleotide Complex Poly(s2C) · poly(I)
Abstract
Poly(s2C) · poly(I) complex formation (where s2C = 2‐thiocytidylate) was shown by spectrophotometric titration, absorption‐difference spectroscopy and hydroxyl‐ion titration. The absorption temperature profile of the complex does not display a transition between 10 and 100°C. Cooperative melting with a transition midpoint at 77°C was observed only after destabilisation by 30% ethyleneglycol. A stabilizing effect of s2CMP residues in hellces is further shown by the observation that 25% s2CMP in poly(C3,s2C) · poly(I) significantly increased the thermal stability compared to poly(C) · poly(I). The ultraviolet absorption spectrum, first derivative absorption spectrum and circular dichroism spectrum suggest a weak n—π* transition at 320 nm in poly(s2C) · poly(I). Unlike other nucleoside diphosphates the polymerisation of s2CDP by polynucleotide phosphorylase from Escherichia coli occurred with a pronounced lag phase which disappeared after the addition of oligonucleotide primers.
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