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A chicory cytochrome P450 mono‐oxygenase CYP71AV8 for the oxidation of (+)‐valencene
Abstract
Chicory (Cichorium intybus L.), which is known to have a variety of terpene‐hydroxylating activities, was screened for a P450 mono‐oxygenase to convert (+)‐valencene to (+)‐nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co‐expression of the enzyme with a valencene synthase in yeast, led to formation of trans‐nootkatol, cis‐nootkatol and (+)‐nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra‐1(10),4,11(13)‐trien‐12‐oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha‐4,11‐diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio‐specificity on (+)‐valencene compared to germacrene A and amorpha‐4,11‐diene.
Citing Literature
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