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Volume 314, Issue 3 p. 466-470
Full-length article
Free Access

Role of liver-type glucose transporter (GLUT2) in transport across the basolateral membrane in rat jejunum

Ken-ichi Miyamoto

Ken-ichi Miyamoto

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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Toshimitsu Takagi

Toshimitsu Takagi

Laboratory of Cell Biology, Teikoku Seiyaku Co., Sanbonmatsu, Kagawa 567, Japan

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Takeru Fujii

Takeru Fujii

Laboratory of Cell Biology, Teikoku Seiyaku Co., Sanbonmatsu, Kagawa 567, Japan

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Tomoko Matsubara

Tomoko Matsubara

Laboratory of Cell Biology, Teikoku Seiyaku Co., Sanbonmatsu, Kagawa 567, Japan

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Kyoko Hase

Kyoko Hase

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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Yutaka Taketani

Yutaka Taketani

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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Tatsuzo Oka

Tatsuzo Oka

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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Hisanori Minami

Hisanori Minami

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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Yukihiro Nakabou

Yukihiro Nakabou

Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan

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First published: December 21, 1992
Citations: 13
Correspondence address: K.-i. Miyamoto, Department of Nutrition, School of Medicine, University of Tokushima, Kuramoto-Cho3, Tokushima 770, Japan.

Abstract

To obtain information on the regulation of glucose transport across the basolateral membrane (BLM) of intestinal epithelial cells, we measured the number of [3H]cytochalasin B binding sites and the level of liver-type glucose transporter (GLUT2) protein in the BLM in the jejunum of rats (i) with diabetes (ii) given a high-carbohydrate diet or (iii) with experimental hyperglycemia (12 h infusion of a high-glucose solution). A glucose uptake and the number of d-glucose inhibitable [3H]cytochalasin B binding sites in BLM vesicles were significantly increased in all three conditions. Western blot analysis showed that the amount of GLUT2 protein in BLM vesicles was increased in rats with diabetes and those given a high-carbohydrate diet, but not in those with experimental hyperglycemia. These results suggest that there is a mechanism for rapid regulation of glucose transport in the BLM that does not depend on change in the amount of GLUT2.