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Volume 185, Issue 1 p. 129-134
Research letters
Free Access

3-Hydroxyacyl-CoA epimerase is a peroxisomal enzyme and therefore not involved in mitochondrial fatty acid oxidation

Chin-hung Chu

Chin-hung Chu

Department of Chemistry, The City College of the City University of New York, New York, NY 10031, USA

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Horst Schulz

Horst Schulz

Department of Chemistry, The City College of the City University of New York, New York, NY 10031, USA

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First published: June 03, 1985
Citations: 26

Abstract

The subcellular location of 3-hydroxyacyl-CoA epimerase (EC 5.1.2.3) was studied by differential centrifugation and Percoll density gradient centrifugation of a rat liver homogenate. The enzyme was found to be associated with peroxisomes but not with mitochondria. This observation proves that 3-hydroxy-acyl-CoA epimerase does not function in mitochondrial β-oxidation ofpolyunsaturated fatty acids which are degraded by a modified pathway.