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Volume 369, Issue 2-3 p. 326-330
Research letter
Free Access

Molecular cloning and sequence analysis of human preprocathepsin C

Alenka Pariš

Corresponding Author

Alenka Pariš

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

Corresponding author. Fax: (386) (61) 273 594.Search for more papers by this author
Borut Štrukelj

Borut Štrukelj

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

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Jože Pungerčar

Jože Pungerčar

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

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Metka Renko

Metka Renko

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

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Iztok Dolenc

Iztok Dolenc

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

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Vito Turk

Vito Turk

Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, Jamova 39, 61000 Ljubljana, Slovenia

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First published: August 07, 1995
Citations: 68

Abstract

A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.