Volume 316, Issue 2 p. 175-180

Research letters

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Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle

Masaki Nakane,

Masaki Nakane

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

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Harald H.H.W. Schmidt,

Harald H.H.W. Schmidt

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

Present address: Medizinische Universitätsklinik, Klinische Forschergruppe, Josef-Schneider-Str. 2, W-8700 Würzburg, Germany. Search for more papers by this author

Jennifer S. Pollock,

Jennifer S. Pollock

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

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Ulrich Förstermann,

Ulrich Förstermann

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

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Ferid Murad,

Ferid Murad

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

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Masaki Nakane,

Masaki Nakane

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

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Harald H.H.W. Schmidt,

Harald H.H.W. Schmidt

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

Present address: Medizinische Universitätsklinik, Klinische Forschergruppe, Josef-Schneider-Str. 2, W-8700 Würzburg, Germany. Search for more papers by this author

Jennifer S. Pollock,

Jennifer S. Pollock

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

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Ulrich Förstermann,

Ulrich Förstermann

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

Search for more papers by this author

Ferid Murad,

Ferid Murad

Vascular Biology, Abbott Laboratories, Abbott Park, Illinois 60064, USA

Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611, USA

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First published: January 25, 1993

https://doi.org/10.1016/0014-5793(93)81210-Q

Citations: 395

Correspondence address: M. Nakane, D46B, AP10, Abbott Laboratories, Abbott Park, IL 60064, USA. Fax: (1) (708) 938-6107.

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Abstract

Complementary DNA clones corresponding to human brain nitric oxide (NO) synthase have been isolated. The deduced amino acid sequence revealed an overall identity with rat brain NO synthase of about 93% and contained all suggested consensus sites for binding of the co-factors. The cDNA transfected COS-1 cells showed significant NO synthase activity with the typical co-factor requirements. Unexpectedly, messenger RNA levels of this isoform of NO synthase was more abundant in human skeletal muscle than human brain. Moreover, we detected high NO synthase activity and the expressed protein in human skeletal muscle by Western blot analysis, indicating a possible novel function of NO in skeletal muscle.

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Volume316, Issue2

January 25, 1993

Pages 175-180

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