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Volume 296, Issue 1 p. 33-36
Full-length article
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The presence of free D-serine in rat brain

Atsushi Hashimoto

Atsushi Hashimoto

Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

Tsukuba Research Laboratory, Nippon Oil & Fats Co., 5-10 Tokodai, Tsukuba Ibaraki 300-26, Japan

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Toru Nishikawa

Toru Nishikawa

Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

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Tohiski Hayashi

Tohiski Hayashi

Division of Biochemical and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

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Noriko Fujii

Noriko Fujii

Department of Chemistry, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan

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Kaoru Harada

Kaoru Harada

Department of Chemistry, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan

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Takae Oka

Takae Oka

Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

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Kiyohisa Takahashi

Kiyohisa Takahashi

Division of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-I Ogawa-Higashi, Kodaira, Tokyo, Japan

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First published: January 13, 1992
Citations: 380
Correspondence address: Toru Nishikawa, Division of Mental Disorder Research, National Institute of Neuroscience, National Cancer of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187, Japan. Fax: (81) (423) 46 1744.

Abstract

Free amino acid enantiomers in adult rat brain extracts were analysed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23, υmol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.