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Cloning, pharmacological characteristics and expression pattern of the rat GABA_A receptor α₄ subunit

William Wisden

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Anne Herb,

Anne Herb

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Heike Wieland,

Heike Wieland

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Kari Keinänen,

Kari Keinänen

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Present address: Biotechnical Laboratory, VTT, Espoo, Finland Search for more papers by this author

Hartmut Lüddens,

Hartmut Lüddens

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Peter H. Seeburg,

Corresponding Author

Peter H. Seeburg

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Correspondence address: P.H. Seeburg, Laboratory Molecular Neuroendocrinology, ZMBH, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany. Fax: (49) (6221) 565894.Search for more papers by this author

William Wisden,

William Wisden

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Anne Herb,

Anne Herb

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Heike Wieland,

Heike Wieland

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Kari Keinänen,

Kari Keinänen

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Present address: Biotechnical Laboratory, VTT, Espoo, Finland Search for more papers by this author

Hartmut Lüddens,

Hartmut Lüddens

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Peter H. Seeburg,

Corresponding Author

Peter H. Seeburg

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Correspondence address: P.H. Seeburg, Laboratory Molecular Neuroendocrinology, ZMBH, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany. Fax: (49) (6221) 565894.Search for more papers by this author

First published: September 09, 1991

https://doi.org/10.1016/0014-5793(91)81076-K

Citations: 213

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Abstract

A cDNA of rat brain encoding the GABA_A receptor α₄ subunit has been cloned. Recombinant receptors composed of α₄, β₂ and γ₂ subunits bind with high affinity the GABA agonist [³H]muscimol and the benzodiazepine ‘alcohol antagonist’ [³H]Ro 15-4513, but fail to bind benzodiazepine agonists. The α₄ subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABA_A receptors. The α₄ mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.

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Volume289, Issue2

September 09, 1991

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Cloning, pharmacological characteristics and expression pattern of the rat GABA_A receptor α₄ subunit

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Cloning, pharmacological characteristics and expression pattern of the rat GABA_A receptor α₄ subunit