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Volume 289, Issue 2 p. 227-230
Full-length article
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Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor α4 subunit

William Wisden

William Wisden

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Anne Herb

Anne Herb

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Heike Wieland

Heike Wieland

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Kari Keinänen

Kari Keinänen

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Present address: Biotechnical Laboratory, VTT, Espoo, Finland Search for more papers by this author
Hartmut Lüddens

Hartmut Lüddens

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

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Peter H. Seeburg

Corresponding Author

Peter H. Seeburg

Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany

Correspondence address: P.H. Seeburg, Laboratory Molecular Neuroendocrinology, ZMBH, Im Neuenheimer Feld 282, D-6900 Heidelberg, Germany. Fax: (49) (6221) 565894.Search for more papers by this author
First published: September 09, 1991
Citations: 211

Abstract

A cDNA of rat brain encoding the GABAA receptor α4 subunit has been cloned. Recombinant receptors composed of α4, β2 and γ2 subunits bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine ‘alcohol antagonist’ [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The α4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The α4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.