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Volume 284, Issue 2 p. 155-160
Full-length article
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Molecular cloning of a novel putative G-protein coupled receptor expressed during rat spermiogenesis

Wolfgang Meyerhof

Wolfgang Meyerhof

Institut für Zellbiochemie und klinische Neurobiologie, UKE, Martinistr. 52, D-2000 Hamburg 20, Germany

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Renate Müller-Brechlin

Renate Müller-Brechlin

Institut für Zellbiochemie und klinische Neurobiologie, UKE, Martinistr. 52, D-2000 Hamburg 20, Germany

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Dietmar Richter

Corresponding Author

Dietmar Richter

Institut für Zellbiochemie und klinische Neurobiologie, UKE, Martinistr. 52, D-2000 Hamburg 20, Germany

Correspondence address: D. Richter, Institut für Zellbiochemic und klinische Neurobiologie, UKE, Martinistr. 52, D-2000 Hamburg 20, Germany. Fax: (49) (40) 4684541.Search for more papers by this author
First published: June 24, 1991
Citations: 147

Abstract

A cDNA clone encoding a novel putative G-protein coupled receptor has been isolated from a rat testis cDNA library using a PCR-amplified cDNA fragment as a hybridization probe. Northern blot analysis reveals that a corresponding 1.5 kb mRNA is exclusively expressed in testis. By in situ hybridization experiments this mRNA has been localized in spermatocytes and spermatids but not in spermatogonia, Leydig or sertoli cells. Ontogenic studies show that expression of the receptor-encoding mRNA and sexual maturation are correlated reaching highest levels during the second and third months. Although the ligand for this receptor has not yet been identified, this receptor may play a role during reproduction.