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Volume 279, Issue 1 p. 110-114
Full-length article
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The oxygenation of cholesterol esters by the reticulocyte lipoxygenase

Jutta Belkner

Jutta Belkner

Institute of Biochemistry, School of Medicine (Charitè), Humboldt University, Hessische Str. 3-4, O-1040 Berlin, Germany

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Rainer Wiesner

Rainer Wiesner

Institute of Biochemistry, School of Medicine (Charitè), Humboldt University, Hessische Str. 3-4, O-1040 Berlin, Germany

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Hartmut Kühn

Hartmut Kühn

Institute of Biochemistry, School of Medicine (Charitè), Humboldt University, Hessische Str. 3-4, O-1040 Berlin, Germany

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Vadim Z. Lankin

Vadim Z. Lankin

All-Union Cardiological Research Center, Academy of Medical Sciences of the USSR, Moscow 12 1552, 3 Cherepkavskaja ul. 15A, USSR

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First published: February 11, 1991
Citations: 62
Correspondence address: H. Kühn, Institute of Biochemistry, Medical School (Charité), Humboldt University, Hessische Str. 3-4, O-1040 Berlin, Germany

Abstract

The arachidonate 15-lipoxygenase from rabbit reticulocytes oxygenates cholesterol esters containing polyenoic fatty acids. Cholesterol esterified with saturated fatty acids is not oxygenated. The structures of the oxygenation products formed from various cholesterol esters have been identified by high pressure liquid chromatography, UV-spectroscopy and gas chromatography/mass spectroscopy. Oxygenated cholesterol esters have been detected in atherosclerotic plaques of human aortas.