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Volume 287, Issue 1-2 p. 23-26
Research letters
Free Access

Cloning and expression of human endothelin-1 receptor cDNA

Kiminori Hosoda

Kiminori Hosoda

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Kazuwa Nakao

Kazuwa Nakao

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Hiroshi-Arai

Hiroshi-Arai

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

Institute for Immunology, Kyoto University School of Medicine, Kyoto 606, Japan

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Shin-ichi Suga

Shin-ichi Suga

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Yoshihiro Ogawa

Yoshihiro Ogawa

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Masashi Mukoyama

Masashi Mukoyama

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Gotaro Shirakami

Gotaro Shirakami

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Yoshihiko Saito

Yoshihiko Saito

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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Shigetada Nakanishi

Shigetada Nakanishi

Institute for Immunology, Kyoto University School of Medicine, Kyoto 606, Japan

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Hiroo Imura

Hiroo Imura

Second Division, Department of Medicine Kyoto University School of Medicine, Kyoto 606, Japan

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First published: August 05, 1991
Citations: 215
Correspondence address: K. Nakao, Second Division, Department of Medicine, Kyoto University School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606, Japan. Fax: (81) (75) 771 9452.

Abstract

We isolated a human endothelin-1 (ET-1) receptor cDNA from a human placenta cDNA library. The cDNA encodes a 427-amino acid protein with seven putative transmembrane domains. The rank order of the binding to the receptor expressed in COS-7 cells was: ET-1 ⩾ ET-2 ⪢ ET-3. The receptor expressed in Xenopus oocytes showed a potent electrophysiological response to 1 × 10−7 M ET-1 under voltage clamp at −60 mV, while a much weaker response was produced by 1 × 10−7 M ET-3. Northern blot analysis with RNA from human tissues revealed a single band with a size of 4.3 kb in a wide variety of human tissues, especially highly in the blood vessel.