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Volume 268, Issue 1 p. 227-230
Research letters
Free Access

Arachidonic acid epoxygenase

Stereochemical analysis of the endogenous epoxyeicosatrienoic acids of human kidney cortex

Armando Karara

Armando Karara

Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA

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Elizabeth Dishman

Elizabeth Dishman

Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA

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Harry Jacobson

Harry Jacobson

Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA

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J.R. Falck

J.R. Falck

Department of Molecular Genetics, University of Texas, Southwestern Medical Center, Dallas, TX 75235, USA

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Jorge H. Capdevila

Jorge H. Capdevila

Division of Nephrology, Vanderbilt University Medical School, Nashville, TN 37232, USA

Department of Biochemistry, Vanderbilt University Medical School, Nashville, TN 37232, USA

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First published: July 30, 1990
Citations: 56
Correspondence address: J.H. Capdevila, Medical Center North S-3223, Vanderbilt University, Nashville, TN 37232, USA.

Abstract

Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid.