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Volume 250, Issue 2 p. 536-540
Full-length article
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CD3/T-cell receptor coupling to a pertussis and cholera toxin-insensitive G-protein

Anders Kvanta

Anders Kvanta

Dept of Pharmacology Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

Dept of Immunology, Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

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Christer Nordstedt

Christer Nordstedt

Dept of Pharmacology Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

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Ingeborg van der Ploeg

Ingeborg van der Ploeg

Dept of Pharmacology Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

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Mikael Jondal

Mikael Jondal

Dept of Immunology, Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

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Bertil B. Fredholm

Bertil B. Fredholm

Dept of Pharmacology Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

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First published: July 03, 1989
Citations: 9
A. Kvanta, Dept of Pharmacology, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden

Abstract

We have analyzed the effect of CD3/T-cell receptor stimulation on GTP hydrolysis and GTP binding. We show that stimulation of Jurkat, T-cell, membranes withT3 results in a 50% increase in GTP hydrolysis which is specifically inhibited by GDP. Pretreatment of the membranes with neither pertussis toxin nor cholera toxin inhibited the GTP hydrolysis. We also show that stimulation withT3 increases the binding of GTPγS to Jurkat membranes. These data strongly implicate the involvement of a G-protein in CD3/T-cell receptor signalling.