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Volume 213, Issue 2 p. 448-452
Research letters
Free Access

The molecular basis of the inherited deficiency of androsterone UDP-glucuronyltransferase in Wistar rats

Robert B. Corser

Robert B. Corser

Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, Scotland

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Michael W.H. Coughtrie

Michael W.H. Coughtrie

Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, Scotland

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Michael R. Jackson

Michael R. Jackson

Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, Scotland

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Brian Burchell

Corresponding Author

Brian Burchell

Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, Scotland

Correspondence address: B. Burchell, Dept of Biochemistry, The University, Dundee DD1 4HN, Scotland.Search for more papers by this author
First published: March 23, 1987
Citations: 20

Abstract

A major UDP-glucurnoyltransferase isoenzyme in rat liver (51 kDa), corresponding to androsterone glucuronidating activity, has been identified by immunoblot analysis. This isoenzyme is absent from Wistar rats exhibiting the low androsterone (LA) UDP-glucuronyltransferase activity phenotype. Northern blot analysis of total RNA from normal and androsterone glucuronidation deficient Wistar rats demonstrated that the mRNA encoding this protein was not synthesised. Differences in restriction fragment length observed on Southern blotting of genomic DNA from LA Wistar rats indicate that this inherited deficiency is the result of a deletion in the androsterone UDP-glucuronyltransferase gene.