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Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus

Corresponding Author

S.D. Trakhanov

Institute of Crystallography, Academy of Sciences of the USSR, Moscow, USSR

Correspondence address: S.D. Trakhanov, Institute of Crystallography, Academy of Sciences of the USSR, Moscow, USSRSearch for more papers by this author

M.M. Yusupov,

M.M. Yusupov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.C. Agalarov,

S.C. Agalarov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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M.B. Garber,

M.B. Garber

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.N. Ryazantsev,

S.N. Ryazantsev

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.V. Tischenko,

S.V. Tischenko

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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V.A. Shirokov,

V.A. Shirokov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.D. Trakhanov,

Corresponding Author

S.D. Trakhanov

Institute of Crystallography, Academy of Sciences of the USSR, Moscow, USSR

Correspondence address: S.D. Trakhanov, Institute of Crystallography, Academy of Sciences of the USSR, Moscow, USSRSearch for more papers by this author

M.M. Yusupov,

M.M. Yusupov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.C. Agalarov,

S.C. Agalarov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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M.B. Garber,

M.B. Garber

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.N. Ryazantsev,

S.N. Ryazantsev

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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S.V. Tischenko,

S.V. Tischenko

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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V.A. Shirokov,

V.A. Shirokov

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region USSR

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First published: August 17, 1987

https://doi.org/10.1016/0014-5793(87)80838-4

Citations: 79

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Abstract

Well-ordered three-dimensional crystals of 70 S ribosomes and 30 S ribosomal subunits from extremely thermophilic bacteria Thermus thermophilus have been obtained. Positively stained thin sections of the crystals have been analyzed by electron microscopy. Redissolved crystalline ribosomes and small ribosomal subunits reveal sedimentation constants of 70 S and 30 S, respectively, and are functionally active in the poly(U)-system.

References

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Volume220, Issue2

August 17, 1987

Pages 319-322

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Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus

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Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus

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