In 2018, we are celebrating the 50th anniversary of FEBS Letters! In its year of launch, FEBS Letters featured only 144 articles. Since then, the journal has grown considerably and has witnessed the publication of several landmark papers. To mark the occasion, we have collected 50 excellent FEBS Letters articles in a Virtual Issue of historical value. We invite you to explore the past, present, and future of Biochemistry and Molecular Biology by taking a closer look at our collection. We hope you enjoy it, and are looking forward to receiving further excellent articles in the near future!